How to Order听

BEFORE YOU PLACE YOUR ORDER, PLEASE READ THE FOLLOWING STEPS:

1) PLEASE read the FAQ below as they contain important ordering and shipping policies.

2) If this is your first order, please contact nxrorders@mbl.edu for a New Account Form

3) Review the Pricing below and the Frog Stock page and contact听nxrorders@mbl.edu听for availability and additional details.

FAQ

QA #1 鈥 Is there an order form for me to fill out?

No. Once you have your 小蓝视频 account set up, e-mail nxrorders@mbl.edu听include your PI name, shipping address, and the details of your frog order (what line you want, what gender, and how many frogs). We will then contact you to finalize your order and arrange a convenient shipping date.

QA #2 鈥 How does the 小蓝视频 invoice?

Invoices are generated by the 小蓝视频鈥檚 Accounts Receivable department at the close of each month and sent via postal mail to the address you provide on your account form. If you require a purchase order (PO) number on your invoice, it must be provided at the time your order is placed.

QA #3 鈥 How can I pay?

Payment terms are net 30. Instruction to submit payment via Check, Bank Transfer or Credit Card will be on the invoice.

QA #4 鈥 Are there certain days (or months, i.e.听Xenopus听tropicalis) the NXR ships orders out?

For domestic orders, we ship frogs out on Mondays, Tuesday, and in some cases Wednesday. For international orders, we ship on Monday or Tuesday. 鈥**Please note we do not ship live frogs if the temperature听is below 40 F at any point along the shipment route for the safety of the animals. This typically means orders placed from December to late February may be delayed.

QA #5 鈥 What method of shipping do you use?

For domestic orders, we use Fed EX and ship next day air. At the time of your order we will ask that you provide us with your institutes Fed EX account so that it will be billed direct. Your order should arrive by 12 noon the next day.听For international orders we use Validated Courier, who are specialists in live animal shipping. This will also be billed directly through an account they will set up with you before the time of shipment.

QA #6 鈥 Is there a health report available for the frogs?

Yes. There are two options for a health report. We can send you a copy of our quarterly qPCR health testing results. Or, for a $97 fee, we can arrange for our vet to inspect the frogs we are sending to you, and furnish you with a Veterinary Health Certificate.

QA #7 鈥 Who is the veterinarian at the NXR?

The NXR consults with the 小蓝视频 veterinarian for frog care issues (and for Veterinary Health Certificates). The current 小蓝视频 vet is Lisa Abbo.

QA #8 鈥 Why do you need a Grant Reference on the New Customer Account form?听

The NXR is funded by an award from the National Institute of Health, Office of the Director, Office of Research Infrastructure Programs (ORIP) and from the Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD). The grant number that you are using to pay for the NXR resources/services is critical information when reporting to the NIH who is benefiting from this public resource.

QA #9 鈥 What is the shipping materials charge?

The shipping materials charge covers the cost of the Styrofoam and plastic boxes used to ship the frogs. The shipping materials are charged at cost, and so the cost varies depending on which species, and how many frogs, you order.

QA #10 鈥 Is an MTA required?

An MTA may be required and we are currently updating that form. We will contact you if it is needed.

QA #11 鈥 Does the NXR accept frogs from my lab that I can no longer use/house?

If you are interested in donating a line to the NXR, please contact us at听xenopus@mbl.edu. We are not always able to accept frogs as our quarantine space is limited.

QA #12 鈥 What is included in your 鈥淩esearch Facility Services鈥?

The Research Facility Services includes lab space, frog use (within reason), common reagents and equipment including picoinjectors, micro manipulators, and a fluorescent stereoscope. If you are interested in using the Research Facility, contact us at听xenopus@mbl.edu听for more details regarding what reagents are available.

Pricing

听齿别苍辞辫耻蝉

Price

J Strain Frogs听X. laevis

$45

Wild-Type Frogs听X. laevis

$45

Wild-Type Frogs听X. laevis听(Oocyte+ Female)

$50

Wild-Type Frogs.听X. tropicalis (Oocyte+ Female)

$45

Wild-Type Frogs. X. tropicalis (Male)听

$50

Mutant/Transgenic Frogs听X. laevis and听X. tropicalis

$55

Wild-Type or Inbred听Embryos听(minimum order of 50 embryos)

$1.00 each

Mutant/Transgenic Embryos听(minimum order of 50 embryos)*

$1.50 each

Wild-Type tadpole stages:听35-47/48-53/53-60/60-65

$1.50 / $2 / $3 / $5

Cryopreserved Sperm 1 vial

$12

Transgenic tadpole stages (screened): 35-47/48-53/53-60/60-65

$2 / $3 / $4 / $6听

Froglets听newly metamorphosed听4-6mo/6-8mo/8-10mo听

$10 / $20 / $30 / $35

听Services

Pricing

听Custom Transgenic听Xenopus line**

$1,250

听Custom Knockout听Xenopus听line**

FREE

听Custom CRISPR sgRNA Construct

$100

听Research Facility Service***

$500/Week

Tank Rentals

Pricing

听Large Tank (40L)

$14.00/tank per day

听Small Tank (23L)

$7.00/tank per day

*A surcharge of $55 for a transgenic male, $40 for a Wild-Type听X. laevis听male, or $50 for a Wild-Type听X. tropicalis听male is added to the order.听If screening of transgenic embryos is requested, a $25 surcharge will be added.

**For more information on custom lines please听contact xenopus@mbl.edu

***Includes lab space, frogs, and common reagents